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Countercurrent chromatography (CCC) is essentially a form of liquid-liquid partition chromatography in which the stationary liquid phase is retained in the apparatus without the use of a solid porous or adsorptive matrix. The principle behind this method of separation involves partitioning of a solute between two immiscible liquid phases, and the relative ratio of the amount of solute passing into each of the two phases is determined by the respective partition coefficient (K). A brief list of the main advantages of the technique includes versatility; speed; high loading capacity; good resolution; economy; predictability and reproducibility; and total sample recovery without chemical modifications or loss of biological activity in bioassay-guided fractionations.