Standardization of red onion methanolic extract to detection of phosphorylated proteins in sds-page
DOI:
https://doi.org/10.3407/rpn.v5i2.6909Keywords:
Antioxidants, flavonoids, fruits, protein carbonylationAbstract
Protein phosphorylation is a reversible post-translational modification, associated with countless vital cell processes such as signal transduction, cell differentiation, development, cell cycle control and metabolic pathway regulation.
References
. Lee, C.R., et al., Determination of protein phosphorylation by polyacrylamide gel electrophoresis. J Microbiol, 2019. 57(2): p. 93-100
. Ardito, F., et al., The crucial role of protein phosphorylation in cell signaling and its use as targeted therapy (Review). Int J Mol Med, 2017. 40(2): p. 271-280.
. Wang, X., et al., Phosphoprotein staining for sodium dodecyl sulfate-polyacrylamide gel electrophoresis using fluorescent reagent morin hydrate. Anal Biochem, 2013. 435(1): p. 19-26.
. Wang, X., et al., Simple detection of phosphoproteins in SDS-PAGE by quercetin. EuPA Open Proteomics, 2014. 4: p. 156-164.
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